6LYK
Crystal Structure of R1263A mutant of Formylglycinamidine Synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2018-07-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 65 |
| Unit cell lengths | 146.900, 146.900, 141.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.740 - 1.700 |
| R-factor | 0.1537 |
| Rwork | 0.152 |
| R-free | 0.17710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t3t |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.208 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.596 | |
| Number of reflections | 188832 | 29675 |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6 | |
| CC(1/2) | 0.959 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2M Ammonium Sulphate |
