6LY7
PylRS C-terminus domain mutant bound with 1-Formyl-L-tryptophan and AMPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2017-03-27 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 64 |
Unit cell lengths | 105.346, 105.346, 72.396 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.355 - 2.094 |
R-factor | 0.173943199964 |
Rwork | 0.173 |
R-free | 0.18385 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zce |
RMSD bond length | 0.010 |
RMSD bond angle | 1.103 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.355 | 2.180 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmeas | 0.104 | 0.829 |
Rpim | 0.039 | 0.308 |
Number of reflections | 26941 | 1908 |
<I/σ(I)> | 33.2 | 2.5 |
Completeness [%] | 96.9 | |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES, 10% PEG 8000, 10% Ethylene Glycerol |