6LTS
Crystal structure of Thermus thermophilus transcription initiation complex comprising a truncated sigma finger
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-09 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.698, 103.847, 295.410 |
| Unit cell angles | 90.00, 98.61, 90.00 |
Refinement procedure
| Resolution | 44.230 - 3.450 |
| R-factor | 0.2209 |
| Rwork | 0.220 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g7h |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.006 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.510 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.165 | 0.695 |
| Number of reflections | 72642 | 3627 |
| <I/σ(I)> | 8.514 | 1.757 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.7 | 295 | 100 mM Tris-HCl, pH 8.7, 200 mM potassium chloride, 50 mM magnesium chloride, 10% PEG4000 |
