6LTQ
Crystal structure of pyrrolidone carboxyl peptidase from thermophilic keratin degrading bacterium Fervidobacterium islandicum AW-1 (FiPcp)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-09-22 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.035, 86.610, 93.529 |
Unit cell angles | 90.00, 105.18, 90.00 |
Refinement procedure
Resolution | 28.288 - 1.850 |
R-factor | 0.1763 |
Rwork | 0.174 |
R-free | 0.22350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2df5 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.880 |
High resolution limit [Å] | 1.845 | 5.020 | 1.850 |
Rmerge | 0.055 | 0.039 | 0.614 |
Rmeas | 0.059 | 0.042 | 0.659 |
Rpim | 0.022 | 0.017 | 0.239 |
Number of reflections | 80445 | 3923 | 3982 |
<I/σ(I)> | 10.5 | ||
Completeness [%] | 99.7 | 94.3 | 100 |
Redundancy | 7.4 | 6.6 | 7.5 |
CC(1/2) | 0.998 | 0.905 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100mM Bis Tris Hydrochloric acid pH 5.5, 200mM Ammonium sulfate |