6LSH
Crystal structure of the enterovirus 71 polymerase elongation complex (C2S6M form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.878, 77.380, 155.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.900 - 2.231 |
R-factor | 0.2098 |
Rwork | 0.208 |
R-free | 0.24450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f8l |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (v1.14) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.310 |
High resolution limit [Å] | 2.230 | 4.800 | 2.230 |
Rmerge | 0.045 | 0.028 | 0.503 |
Rmeas | 0.049 | 0.031 | 0.576 |
Rpim | 0.021 | 0.013 | 0.272 |
Total number of observations | 196215 | ||
Number of reflections | 35885 | 3952 | 2929 |
<I/σ(I)> | 13 | ||
Completeness [%] | 95.2 | 98.4 | 79.1 |
Redundancy | 5.5 | 5.4 | 3.9 |
CC(1/2) | 0.999 | 0.843 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 289 | MES, PEG 5000 monomethyl ether, glycerol |