6LSF
Crystal structure of the enterovirus 71 polymerase elongation complex (C2S6RA/C2S6RB form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.350, 76.447, 155.004 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.729 - 2.152 |
R-factor | 0.2169 |
Rwork | 0.215 |
R-free | 0.25060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f8l |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
Rmerge | 0.067 | 0.049 | 0.408 |
Rmeas | 0.076 | 0.055 | 0.466 |
Rpim | 0.034 | 0.025 | 0.220 |
Total number of observations | 170510 | ||
Number of reflections | 36176 | 3962 | 2655 |
<I/σ(I)> | 12.1 | ||
Completeness [%] | 89.5 | 92.1 | 66.8 |
Redundancy | 4.7 | 4.7 | 3.7 |
CC(1/2) | 0.995 | 0.878 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 289 | MES, PEG 5000 monomethyl ether, glycerol |