6LSE

Crystal structure of the enterovirus 71 polymerase elongation complex (C3S6A/C3S6B form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	62.607, 77.015, 156.679
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.580 - 2.250
R-factor	0.2013
Rwork	0.199
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f8l
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.330
High resolution limit [Å]	2.250	4.850	2.250
Rmerge	0.082	0.058	0.519
Rmeas	0.092	0.066	0.595
Rpim	0.040	0.029	0.283
Total number of observations	140354
Number of reflections	32014	3401	2717
<I/σ(I)>	8.6
Completeness [%]	87.2	86.9	75.2
Redundancy	4.4	4.4	3.6
CC(1/2)		0.994	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	289	MES, PEG 5000 monomethyl ether, glycerol