6LR3
Structural and functional insights into macrophage migration inhibitory factor from Oncomelania hupensis, the intermediate host of Schistosoma japonicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.302, 139.479, 105.672 |
| Unit cell angles | 90.00, 89.81, 90.00 |
Refinement procedure
| Resolution | 49.350 - 1.770 |
| R-factor | 0.1517 |
| Rwork | 0.150 |
| R-free | 0.17970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lkv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.596 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.074 | 0.242 |
| Number of reflections | 135295 | 6270 |
| <I/σ(I)> | 19 | 4.6 |
| Completeness [%] | 96.2 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 5% v/v 2-Propanol, and 2.0 M Ammonium sulfate |
