6LPF

The crystal structure of human cytoplasmic LRS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	97
Detector technology	PIXEL
Collection date	2017-05-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97775
Spacegroup name	C 2 2 21
Unit cell lengths	88.217, 94.684, 680.055
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.077 - 2.490
R-factor	0.2007
Rwork	0.199
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wz2
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.490	6.780	2.500
Rmerge	0.088	0.035	0.704
Rmeas	0.094	0.037	0.767
Rpim	0.030	0.012	0.297
Number of reflections	100132	5378	4772
<I/σ(I)>	6.6
Completeness [%]	99.9	99.9	98.6
Redundancy	9.5	9.4	6.2
CC(1/2)		0.997	0.723

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	20% (w/v) PEG 6000, 100 mM Tris-cl, pH 8.0, and 200 mM Lithium chloride