6LOJ
The complex structure of IpaH9.8-LRR and hGBP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 122.490, 122.490, 582.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.910 - 3.720 |
R-factor | 0.2287 |
Rwork | 0.227 |
R-free | 0.25550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f5n |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.910 | 3.853 |
High resolution limit [Å] | 3.720 | 3.720 |
Number of reflections | 28447 | 2754 |
<I/σ(I)> | 12.52 | 2.59 |
Completeness [%] | 99.7 | 99.38 |
Redundancy | 37.3 | 12.1 |
CC(1/2) | 0.996 | 0.775 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 2.5M NaCl, 0.1M K/Na phosphate buffer, pH 6 |