6LOI
Crystal structure of Enterococcus faecalis Undecaprenyl pyrophosphate synthase(EfaUPPS)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 31 |
Unit cell lengths | 82.779, 82.779, 213.706 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.351 - 2.503 |
R-factor | 0.1803 |
Rwork | 0.178 |
R-free | 0.23820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ueh |
Data reduction software | HKL-3000 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.472 | |
Rmeas | 0.539 | |
Rpim | 0.269 | |
Number of reflections | 54407 | 1926 |
<I/σ(I)> | 1.2 | 1.2 |
Completeness [%] | 96.5 | 69 |
Redundancy | 6.6 | 3 |
CC(1/2) | 0.993 | 0.778 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M citrate acid/sodium hydroxide (pH 5.0), 20% (w/v) PEG 6000 |