6LN4

Estrogen-related receptor beta(ERR2) in complex with PGC1a-2a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-04-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	108.472, 108.472, 106.902
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.370 - 2.610
R-factor	0.2005
Rwork	0.198
R-free	0.24480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e2r
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.610
High resolution limit [Å]	2.570	6.970	2.570
Rmerge	0.141	0.078	0.657
Rmeas	0.144	0.080	0.685
Rpim	0.029	0.018	0.182
Total number of observations	309875
Number of reflections	12633	739	592
<I/σ(I)>	6.1
Completeness [%]	99.8	99.9	97.4
Redundancy	24.5	20.5	11
CC(1/2)		0.999	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100mM Kcl, 20% PEG 4000