6LLB
Crystal structure of mpy-RNase J (mutant S247A), an archaeal RNase J from Methanolobus psychrophilus R15, in complex with 6 nt RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2018-09-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 168.726, 168.726, 166.427 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.746 - 2.600 |
R-factor | 0.1897 |
Rwork | 0.188 |
R-free | 0.22430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ws2 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.710 | 48.710 | 2.700 |
High resolution limit [Å] | 2.600 | 9.730 | 2.600 |
Rmerge | 0.082 | 0.018 | 0.938 |
Rmeas | 0.085 | 0.019 | 0.969 |
Rpim | 0.021 | 0.005 | 0.242 |
Total number of observations | 671046 | 11913 | 70100 |
Number of reflections | 43478 | 968 | 4459 |
<I/σ(I)> | 31.7 | 105.7 | 4.2 |
Completeness [%] | 100.0 | 99.2 | 100 |
Redundancy | 15.4 | 12.3 | 15.7 |
CC(1/2) | 1.000 | 1.000 | 0.933 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.01 M MgSO4, 0.05 M (CH3)2AsO2Na (pH 6.5) and 2 M (NH4)2SO4. |