6LKW
Structural and functional insights into macrophage migration inhibitory factor from Oncomelania hupensis, the intermediate host of Schistosoma japonicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97855 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 172.842, 172.842, 172.842 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.950 - 3.200 |
| R-factor | 0.2352 |
| Rwork | 0.230 |
| R-free | 0.33310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bsi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.543 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.100 | 0.596 |
| Number of reflections | 15176 | 727 |
| <I/σ(I)> | 55.33 | 10 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 75.7 | 65.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 30% (w/v) MPD, 0.1 M Sodium acetate (pH 4.6), and 20 mM Calcium chloride |
