6LKS
Effects of zinc ion on oligomerization and pH stability of influenza virus hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 215.710, 124.270, 214.530 |
| Unit cell angles | 90.00, 102.91, 90.00 |
Refinement procedure
| Resolution | 48.750 - 3.240 |
| R-factor | 0.2661 |
| Rwork | 0.265 |
| R-free | 0.29480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4edb |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.671 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.750 | 3.356 |
| High resolution limit [Å] | 3.240 | 3.240 |
| Rmeas | 0.287 | 0.728 |
| Rpim | 0.078 | 0.206 |
| Number of reflections | 172506 | 8830 |
| <I/σ(I)> | 7.73 | 3.23 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.3 | |
| CC(1/2) | 0.988 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 297 | PEG 2000, Tris, DMSO |
