Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.585, 178.935, 107.279 |
| Unit cell angles | 90.00, 106.70, 90.00 |
Refinement procedure
| Resolution | 47.490 - 2.100 |
| Rwork | 0.188 |
| R-free | 0.22280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iph |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.073 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.490 | 2.175 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.064 | 0.485 |
| Number of reflections | 156082 | 15567 |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 97.1 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.994 | 0.643 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M sodium cacodylate, 40% MPD, PEG8000 5% |
