6LFF
transcription factor SATB1 CUTr1 domain in complex with a phosphorothioate DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2015-05-30 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 |
| Unit cell lengths | 45.320, 45.320, 97.862 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.630 - 1.790 |
| R-factor | 0.23207 |
| Rwork | 0.230 |
| R-free | 0.27122 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o4a |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.224 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.940 | 1.890 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.105 | 0.353 |
| Number of reflections | 19724 | 3103 |
| <I/σ(I)> | 11.6 | 4.8 |
| Completeness [%] | 93.2 | 100 |
| Redundancy | 6.7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 50 mM Tris-HCl (pH 8.5), 20% polyethylene glycol monomethyl ether 550 (Sigma-Aldrich), 20% ethylene glycol, and 10 mM MgCl2 |
