6LF8
Crystal structure of pSLA-1*0401 complex with dodecapeptide RVEDVTNTAEYW
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 217.150, 45.280, 44.900 |
| Unit cell angles | 90.00, 90.45, 90.00 |
Refinement procedure
| Resolution | 108.570 - 2.500 |
| R-factor | 0.25175 |
| Rwork | 0.250 |
| R-free | 0.28513 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qq3 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.570 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.141 | |
| Rpim | 0.078 | |
| Number of reflections | 19715 | 4296 |
| <I/σ(I)> | 12.1 | 6.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M Bis-Tris pH 6.5, 25% w/v Polyethylene Glycol 3350 |
