6LES
3D domain-swapped dimer of the maltose-binding protein fused to a fragment of the focal adhesion kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-13 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.980 |
Spacegroup name | P 1 |
Unit cell lengths | 65.910, 71.960, 83.390 |
Unit cell angles | 97.84, 90.03, 106.55 |
Refinement procedure
Resolution | 47.450 - 2.004 |
R-factor | 0.2015 |
Rwork | 0.200 |
R-free | 0.23050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5bmy |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.490 | 2.076 |
High resolution limit [Å] | 2.000 | 2.004 |
Rmerge | 0.103 | 0.944 |
Rmeas | 0.123 | |
Rpim | 0.066 | 0.723 |
Number of reflections | 93102 | 8178 |
<I/σ(I)> | 8.07 | 1.2 |
Completeness [%] | 95.3 | 84.49 |
Redundancy | 8.12 | 1.2 |
CC(1/2) | 0.995 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 298 | 2000mM Ammonium sulfate, 100mM CAPS/ Sodium hydroxide pH 10.5 |