6LD4
Zika NS5 polymerase domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-23 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9538 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.100, 85.170, 69.810 |
Unit cell angles | 90.00, 113.34, 90.00 |
Refinement procedure
Resolution | 21.910 - 1.500 |
Rwork | 0.196 |
R-free | 0.22380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tfr |
RMSD bond length | 0.013 |
RMSD bond angle | 1.451 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.910 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.042 | 1.250 |
Number of reflections | 109191 | 10777 |
<I/σ(I)> | 11.84 | |
Completeness [%] | 99.2 | |
Redundancy | 3.2 | |
CC(1/2) | 0.999 | 0.381 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 10% PEG 6000, 0.2M HEPES pH7.5 |