6LC9
Crystal structure of AmpC Ent385 complex form with ceftazidime
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.389, 74.083, 102.822 |
| Unit cell angles | 90.00, 94.79, 90.00 |
Refinement procedure
| Resolution | 43.670 - 1.650 |
| R-factor | 0.1642 |
| Rwork | 0.163 |
| R-free | 0.19420 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.928 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.700 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.057 | 0.773 |
| Number of reflections | 89346 | 14132 |
| <I/σ(I)> | 18.84 | |
| Completeness [%] | 98.1 | |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 1.8 M ammonium sulfate, 0.1 M MES pH 5.5 and 5% 1,4-Dioxane |
