6LC8
Crystal structure of AmpC Ent385 complex form with avibactam
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.418, 73.672, 102.180 |
Unit cell angles | 90.00, 94.49, 90.00 |
Refinement procedure
Resolution | 37.600 - 1.600 |
Rwork | 0.167 |
R-free | 0.18900 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.005 |
RMSD bond angle | 0.779 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.500 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.059 | 0.666 |
Number of reflections | 96869 | 15241 |
<I/σ(I)> | 16.86 | |
Completeness [%] | 98.4 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 1.8 M ammonium sulfate, 0.1 M MES pH 5.5 and 5% 1,4-Dioxane |