6LC1
Structural basis of NR4A1 bound to the human pituitary proopiomelanocortin gene promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.822, 63.210, 91.762 |
| Unit cell angles | 109.98, 90.21, 89.54 |
Refinement procedure
| Resolution | 29.646 - 3.120 |
| R-factor | 0.2125 |
| Rwork | 0.209 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cit |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.730 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.646 | 3.232 |
| High resolution limit [Å] | 3.120 | 3.120 |
| Rmeas | 0.136 | 0.357 |
| Rpim | 0.071 | 0.181 |
| Number of reflections | 12751 | 984 |
| <I/σ(I)> | 7 | 2.54 |
| Completeness [%] | 92.1 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Ammonium sulfate, 10mM magnesium chloride, 50mM MES monohydrate pH 5.6, 20% w/v Polyethylene glycol 8K |
