6LBM
Crystal Structure of FOXC2-DBD bound to a palindromic DNA sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.521, 42.656, 103.277 |
Unit cell angles | 90.00, 103.16, 90.00 |
Refinement procedure
Resolution | 39.281 - 2.841 |
R-factor | 0.234 |
Rwork | 0.233 |
R-free | 0.26260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ako |
RMSD bond length | 0.005 |
RMSD bond angle | 0.709 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.281 | 2.940 |
High resolution limit [Å] | 2.840 | 2.840 |
Number of reflections | 9399 | 547 |
<I/σ(I)> | 15.43 | 1.8 |
Completeness [%] | 94.6 | |
Redundancy | 7.1 | |
CC(1/2) | 0.986 | 0.718 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 50 mM HEPES, pH 7.5, 8%-12% PEG4000 (w/v), 250 mM NaCl, 10 mM MgCl2 and 1 mM TCEP. |