6LBI
Crystal Structure of FOXO1-DBD homodimer bound to a palindromic DNA sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.732, 89.090, 194.166 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.793 - 3.067 |
R-factor | 0.2286 |
Rwork | 0.227 |
R-free | 0.26410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3co6 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.467 |
Data reduction software | HKL-2000 (3.25) |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.793 | 3.170 |
High resolution limit [Å] | 3.060 | 3.060 |
Rpim | 0.107 | 1.208 |
Number of reflections | 17379 | 314 |
<I/σ(I)> | 8.8 | |
Completeness [%] | 99.0 | |
Redundancy | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 50 mM NaAc, pH 4.7, 12%-18% PEG4000 (w/v), 200 mM NaCl, 10 mM MgCl2 and 1 mM TCEP. |