6LAZ
the wildtype SAM-VI riboswitch bound to a N-mustard SAM analog M1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-07-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.102 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.390, 85.348, 93.165 |
| Unit cell angles | 90.00, 105.46, 90.00 |
Refinement procedure
| Resolution | 42.670 - 2.760 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.22340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6las |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.181 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.670 | 42.670 | 2.900 |
| High resolution limit [Å] | 2.760 | 8.710 | 2.760 |
| Rmerge | 0.075 | 0.046 | 0.704 |
| Rmeas | 0.081 | 0.050 | 0.761 |
| Rpim | 0.031 | 0.019 | 0.285 |
| Total number of observations | 4665 | 21188 | |
| Number of reflections | 20968 | 722 | 3051 |
| <I/σ(I)> | 15.9 | 33.4 | 2.6 |
| Completeness [%] | 94.8 | 97.7 | 95.4 |
| Redundancy | 6.9 | 6.5 | 6.9 |
| CC(1/2) | 0.998 | 0.998 | 0.885 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M sodium acetate trihydrate pH 4.6, 10% w/v polyethylene glycol 4,000 |
