6LA9

349 bp di-nucleosome harboring cohesive DNA termini assembled with linker histone H1.0 (high cryoprotectant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98.15
Detector technology	PIXEL
Collection date	2017-09-01
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	120.780, 172.880, 216.041
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.780 - 3.700
R-factor	0.1958
Rwork	0.194
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lab 4qlc
RMSD bond length	0.006
RMSD bond angle	1.442
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.010	3.900
High resolution limit [Å]	3.700	3.700
Rmerge	0.212	1.642
Rmeas	0.234
Rpim	0.096	0.796
Number of reflections	47850	6640
<I/σ(I)>	7.8	1.4
Completeness [%]	97.6	94.2
Redundancy	10.6	9
CC(1/2)	0.998	0.309

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291.15	Calcium chloride, potassium chloride, sodium acetate