6L9W
Crystal structure of mouse TIFA (T9E/C36S mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-30 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 149.278, 115.885, 80.938 |
Unit cell angles | 90.00, 106.77, 90.00 |
Refinement procedure
Resolution | 37.290 - 2.900 |
R-factor | 0.2155 |
Rwork | 0.213 |
R-free | 0.26640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6l9u |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.290 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.084 | 0.294 |
Number of reflections | 28175 | 2170 |
<I/σ(I)> | 23.3 | |
Completeness [%] | 95.7 | |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | MES pH 6.4, MPD |