6L9C

Neutron structure of copper amine oxidase from Arthrobacter glibiformis at pD 7.4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	157.548, 61.779, 92.332
Unit cell angles	90.00, 112.13, 90.00

Refinement procedure

Resolution	20.951 - 1.720
R-factor	0.1815
Rwork	0.180
R-free	0.20950
RMSD bond length	0.009
RMSD bond angle	1.318
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.160
High resolution limit [Å]	1.140	3.090	1.140
Rmerge	0.069	0.056	0.801
Rmeas	0.076	0.062	0.950
Rpim	0.029	0.027	0.504
Number of reflections	296297	13805	14901
<I/σ(I)>	14.8
Completeness [%]	99.4	90.6	99.9
Redundancy	5.2	6.3	3.5
CC(1/2)		0.992	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	289	1.05M potassium-sodium tartrate, 25mM HEPES
1	MICRODIALYSIS	7.4	289	1.05M potassium-sodium tartrate, 25mM HEPES