6L84

Complex of DNA polymerase IV and D-DNA duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 2 21 21
Unit cell lengths	52.763, 98.664, 100.769
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.872 - 2.602
R-factor	0.1869
Rwork	0.182
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bq3
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.122	0.118	0.359
Rmeas	0.127	0.123	0.384
Rpim	0.036	0.036	0.133
Number of reflections	26904	932	835
<I/σ(I)>	11.7
Completeness [%]	99.8	99	99.8
Redundancy	11.3	12.4	7.7
CC(1/2)		0.981	0.858

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.1 M BIS-TRIS (pH 7.0), 0.1 M calcium acetate, 2% glycerol, and 9% w/v PEG3350