6L72
Sirtuin 2 demyristoylation native final product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-09-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.736, 77.572, 56.072 |
| Unit cell angles | 90.00, 97.35, 90.00 |
Refinement procedure
| Resolution | 45.197 - 2.501 |
| R-factor | 0.1802 |
| Rwork | 0.176 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y6l |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.094 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 11004 | 551 |
| <I/σ(I)> | 25.1 | 2.86 |
| Completeness [%] | 98.7 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.997 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M MES, pH=6.5, 6%-20% (w/v) PEG10000 |
