6L6S
The structure of the UdgX mutant H109E crosslinked to single-stranded DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.997 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 44.265, 82.348, 122.444 |
Unit cell angles | 90.00, 94.93, 90.00 |
Refinement procedure
Resolution | 40.664 - 2.064 |
R-factor | 0.211757940956 |
Rwork | 0.209 |
R-free | 0.26113 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6iod |
RMSD bond length | 0.011 |
RMSD bond angle | 1.135 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.060 | 2.070 |
Rmerge | 0.050 | 0.570 |
Number of reflections | 26965 | 2675 |
<I/σ(I)> | 34.2 | 4.1 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 6.8 | 6.5 |
CC(1/2) | 0.997 | 0.914 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 2.15 M sodium malonate pH 7.0 and 3.8% MPD |