6L6S
The structure of the UdgX mutant H109E crosslinked to single-stranded DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.997 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 44.265, 82.348, 122.444 |
| Unit cell angles | 90.00, 94.93, 90.00 |
Refinement procedure
| Resolution | 40.664 - 2.064 |
| R-factor | 0.211757940956 |
| Rwork | 0.209 |
| R-free | 0.26113 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6iod |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.135 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.060 | 2.070 |
| Rmerge | 0.050 | 0.570 |
| Number of reflections | 26965 | 2675 |
| <I/σ(I)> | 34.2 | 4.1 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 6.8 | 6.5 |
| CC(1/2) | 0.997 | 0.914 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 2.15 M sodium malonate pH 7.0 and 3.8% MPD |
