6L6P
hASIC2a co-crystallized with Mamb-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 109.863, 155.664, 156.644 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.230 - 3.080 |
| R-factor | 0.2337 |
| Rwork | 0.231 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.539 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.330 | 49.330 | 3.180 |
| High resolution limit [Å] | 3.080 | 12.320 | 3.080 |
| Rmerge | 0.107 | 0.031 | 1.294 |
| Rmeas | 0.117 | 0.035 | 1.412 |
| Rpim | 0.045 | 0.015 | 0.557 |
| Total number of observations | 329259 | 4686 | 28517 |
| Number of reflections | 50203 | 824 | 4543 |
| <I/σ(I)> | 12.6 | 45.6 | 1.5 |
| Completeness [%] | 99.6 | 93 | 99.6 |
| Redundancy | 6.6 | 5.7 | 6.3 |
| CC(1/2) | 0.998 | 0.998 | 0.644 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 4% Tascimate pH 8.0, 12% w/v PEG3350 |
