6L65
Sirtuin 2 protein with H3K18 myristoylated peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-05-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97906 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.062, 73.399, 55.693 |
Unit cell angles | 90.00, 93.86, 90.00 |
Refinement procedure
Resolution | 55.594 - 1.800 |
R-factor | 0.1789 |
Rwork | 0.177 |
R-free | 0.21840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r8m |
RMSD bond length | 0.007 |
RMSD bond angle | 0.955 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.600 | 1.660 |
High resolution limit [Å] | 1.580 | 1.580 |
Number of reflections | 29350 | 1169 |
<I/σ(I)> | 11.7 | |
Completeness [%] | 74.0 | |
Redundancy | 6.06 | |
CC(1/2) | 0.992 | 0.522 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 293.1 | 0.1M MES, pH 6.5, 6%-20% (w/v) PEG 10000 |