6L3Y
Crystal Structure of Lysyl-tRNA Synthetase from Plasmodium falciparum complexed with L-lysine and Clado-C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2019-05-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 299.841, 52.831, 70.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.960 - 3.100 |
R-factor | 0.228 |
Rwork | 0.225 |
R-free | 0.27990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pg3 |
Data reduction software | DIALS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15rc1_3423) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 149.921 | 2.948 |
High resolution limit [Å] | 2.890 | 2.898 |
Number of reflections | 25891 | 1293 |
<I/σ(I)> | 5.7 | 0.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.3 | |
CC(1/2) | 0.989 | 0.446 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.09M NPS-0.3M Sodium nitrate, 0.3 Sodium phosphate dibasic,0.3M Ammonium sulfate. 1.0M Buffer-pH6.5- Imidazole; MES monohydrate (acid) Precipitant Mix-25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350 |