6L3E

Crystal structure of Salmonella enterica sugar-binding protein MalE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	87.714, 89.692, 64.066
Unit cell angles	90.00, 114.77, 90.00

Refinement procedure

Resolution	39.820 - 1.600
R-factor	0.1665
Rwork	0.166
R-free	0.18570
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.821
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.620
High resolution limit [Å]	1.600	1.600
Number of reflections	58520	278
<I/σ(I)>	33.29	2.35
Completeness [%]	99.5
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		279	50% MPD, 0.1 M HEPES (pH 7.5)