6L2I
IlvC, a ketol-acid reductoisomerase, from Streptococcus pneumoniae_WT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-29 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.063, 104.349, 110.942 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.630 - 1.690 |
Rwork | 0.194 |
R-free | 0.20860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1np3 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.087 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.630 | 1.750 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.068 | 0.470 |
Rmeas | 0.071 | 0.486 |
Rpim | 0.019 | 0.126 |
Number of reflections | 88937 | 8757 |
<I/σ(I)> | 58.1 | 6.16 |
Completeness [%] | 99.0 | |
Redundancy | 14.4 | |
CC(1/2) | 0.998 | 0.980 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M HEPES pH 7.5, 0.1M NaCl 1.5 M Amminoum sulfate |