6L0F

Crystal structure of dihydroorotase in complex with 5-Aminouracil from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-16
Detector	RAYONIX MX325HE
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	85.649, 88.739, 103.395
Unit cell angles	90.00, 95.67, 90.00

Refinement procedure

Resolution	34.300 - 3.260
R-factor	0.2027
Rwork	0.200
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l0a
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.300	3.370
High resolution limit [Å]	3.260	3.260
Number of reflections	24080	2299
<I/σ(I)>	8.6
Completeness [%]	99.1
Redundancy	4
CC(1/2)	0.926	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	291	10 mM imidazole malate pH 7.1, 18 % PEG 4000