6KZ8
Crystal structure of plant Phospholipase D alpha complex with phosphatidic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9798 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 99.042, 123.030, 138.904 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.890 - 2.291 |
R-factor | 0.1992 |
Rwork | 0.198 |
R-free | 0.24460 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.890 | 2.370 |
High resolution limit [Å] | 2.290 | 2.300 |
Number of reflections | 101062 | 6209 |
<I/σ(I)> | 27.6 | |
Completeness [%] | 99.8 | |
Redundancy | 12.6 | |
CC(1/2) | 0.992 | 0.785 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG 3000, 0.1M Tris-HCl (pH 7.0), 0.2M Ca(OAc)2 |