6KYT
The structure of the M. tb toxin MazEF-mt1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.697, 161.140, 78.851 |
Unit cell angles | 90.00, 99.63, 90.00 |
Refinement procedure
Resolution | 28.681 - 2.001 |
R-factor | 0.196306485999 |
Rwork | 0.195 |
R-free | 0.22984 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wyg |
RMSD bond length | 0.003 |
RMSD bond angle | 0.635 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.076 | 0.907 |
Number of reflections | 85400 | 8535 |
<I/σ(I)> | 18.1 | 1.3 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 6.8 | 6.6 |
CC(1/2) | 0.995 | 0.698 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 25% PEG1500, 0.1M BIS-Tris pH 9.0, 0.1M NaCl |