5WYG

The crystal structure of the apo form of Mtb MazF

Summary for 5WYG

• Entry DOI: 10.2210/pdb5wyg/pdb
• Descriptor: Probable endoribonuclease MazF7 (2 entities in total)
• Functional Keywords: mycobacterium tuberculosis, toxin-antitoxin system, mazf, hydrolase
• Biological source: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
• Total number of polymer chains: 2
• Total formula weight: 29303.69

Authors

Xie, W.,Chen, R.,Tu, J. (deposition date: 2017-01-13, release date: 2017-04-12, Last modification date: 2023-11-22)

Primary citation

Chen, R.,Tu, J.,Liu, Z.,Meng, F.,Ma, P.,Ding, Z.,Yang, C.,Chen, L.,Deng, X.,Xie, W.
Structure of the MazF-mt9 toxin, a tRNA-specific endonuclease from Mycobacterium tuberculosis
Biochem. Biophys. Res. Commun., 486:804-810, 2017

PubMed Abstract: Tuberculosis (TB) is a severe disease caused by Mycobacterium tuberculosis (M. tb) and the well-characterized M. tb MazE/F proteins play important roles in stress adaptation. Recently, the MazF-mt9 toxin has been found to display endonuclease activities towards tRNAs but the mechanism is unknown. We hereby present the crystal structure of apo-MazF-mt9. The enzyme recognizes tRNA with a central UUU motif within the anticodon loop, but is insensitive to the sequence context outside of the loop. Based on our crystallographic and biochemical studies, we identified key residues for catalysis and proposed the potential tRNA-binding site.

PubMed: 28351618
DOI: 10.1016/j.bbrc.2017.03.132

Experimental method

X-RAY DIFFRACTION (2.356 Å)