6KYR

Crystal structure of DCLK1 mutant (P675L) Autoinhibited Kinase Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	77.941, 78.003, 177.611
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.145 - 2.206
R-factor	0.1819
Rwork	0.179
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fg9
RMSD bond length	0.003
RMSD bond angle	0.668
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.200	2.200
Number of reflections	55177	3594
<I/σ(I)>	16.4
Completeness [%]	100.0
Redundancy	6.3
CC(1/2)		0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.6M NaH2PO4/K2HPO4, PH 7.8