6KYL
Crystal Structure of Phosphatidic acid Transporter Ups1/Mdm35 in Complex with (2R)-3-(phosphonooxy)propane-1,2-diyl dihexanoate
Replaces: 5JQOExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-06 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 0.9786 |
Spacegroup name | P 41 |
Unit cell lengths | 90.140, 90.140, 81.319 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.070 - 3.550 |
R-factor | 0.2892 |
Rwork | 0.287 |
R-free | 0.31030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jql |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.610 |
High resolution limit [Å] | 3.549 | 9.610 | 3.550 |
Rmerge | 0.053 | 0.029 | 0.670 |
Rmeas | 0.057 | 0.032 | 0.724 |
Rpim | 0.022 | 0.013 | 0.273 |
Number of reflections | 8015 | 428 | 407 |
<I/σ(I)> | 6.4 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 6.7 | 6.1 | 6.9 |
CC(1/2) | 0.999 | 0.849 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M Bis-tris Propane, 30%-32% v/v Tacsimate, pH7.0 |