6KYJ

Hybrid-Rubisco (rice RbcL and sorghum RbcS) in complex with sulfate ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-18
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0
Spacegroup name	P 4 21 2
Unit cell lengths	223.790, 223.790, 108.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.580 - 1.700
R-factor	0.1635
Rwork	0.162
R-free	0.18470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3axm
RMSD bond length	0.007
RMSD bond angle	0.852
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.580	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.118	1.138
Rmeas	0.121
Rpim	0.023	0.234
Number of reflections	299765	29707
<I/σ(I)>	23.31	3.13
Completeness [%]	100.0
Redundancy	27.2
CC(1/2)	0.999	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M Tris-HCl (pH 9.0), 0.2M lithium sulfate, 15%(w/v) polyethylene glycol 4000, and 10 %(w/v) glycerol