6KXV
Crystal structure of a nucleosome containing Leishmania histone H3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-14 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 101.903, 101.101, 175.526 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.932 - 3.630 |
R-factor | 0.2225 |
Rwork | 0.220 |
R-free | 0.26390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ay8 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.583 |
Data reduction software | XDS (BUILT=20170615) |
Data scaling software | XSCALE (BUILT=20170615) |
Phasing software | PHASER (2.8.0) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.932 | 48.932 | 3.850 |
High resolution limit [Å] | 3.630 | 10.660 | 3.630 |
Rmerge | 0.232 | 0.060 | 2.226 |
Rmeas | 0.238 | 0.061 | 2.284 |
Number of reflections | 21054 | 926 | 3250 |
<I/σ(I)> | 10.57 | 47.92 | 1.34 |
Completeness [%] | 99.6 | 98.7 | 97.7 |
Redundancy | 20.991 | 18.941 | 20.003 |
CC(1/2) | 0.999 | 0.999 | 0.515 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium acetate, isopropanol, manganese chloride, trimethylamine N-oxide |