6KVF
Structure of anti-hCXCR2 abN48 in complex with its CXCR2 epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.509, 76.082, 85.364 |
| Unit cell angles | 90.00, 108.53, 90.00 |
Refinement procedure
| Resolution | 39.420 - 2.790 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.26440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xcn |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.762 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.790 | 2.800 |
| Number of reflections | 24116 | 2817 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 6.7 | 6.5 |
| CC(1/2) | 1.000 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES sodium pH 7.5, 2% v/v Polyethylene glycol 400, 2.0M Ammonium sulfate |
