6KUY
Crystal structure of the alpha2A adrenergic receptor in complex with a partial agonist
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.210, 71.620, 286.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.620 - 3.200 |
| R-factor | 0.2683 |
| Rwork | 0.267 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y02 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.628 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.100 | 0.550 |
| Number of reflections | 11252 | 11252 |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 90.5 | 90.5 |
| Redundancy | 10.7 | 6.3 |
| CC(1/2) | 0.998 | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 0.1M HEPES sodium pH 7.4, 310mM Ammonium tartrate dibasic, 36.5% PEG400 |
