6KU3

Crystal structure of gibberellin 2-oxidase3 (GA2ox3)in rice

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-31
Detector	RAYONIX MX-225
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	99.470, 112.739, 149.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.876 - 2.150
R-factor	0.1992
Rwork	0.197
R-free	0.24310
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.190
High resolution limit [Å]	2.150	5.830	2.150
Rmerge	0.093	0.062	0.618
Rmeas	0.104	0.071	0.689
Rpim	0.047	0.034	0.303
Total number of observations	456613
Number of reflections	91795	4821	4540
<I/σ(I)>	7.8
Completeness [%]	99.9	98	100
Redundancy	5	4.4	4.9
CC(1/2)		0.993	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium sulfate, 0.1 M Tris-HCl (pH 8.5) and 12% (w/v) PEG8000