6KTC

Crystal structure of YBX1 CSD with m5C RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9789
Spacegroup name	P 62
Unit cell lengths	66.706, 66.706, 34.224
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.353 - 2.008
R-factor	0.2388
Rwork	0.237
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i2z
RMSD bond length	0.003
RMSD bond angle	0.647
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	5075	265
<I/σ(I)>	17.4	4.7
Completeness [%]	97.8	86.9
Redundancy	13.2	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	2.5 M Ammonium sulfate, 0.1 M Tris pH 8.5.