6KRV
Crystal structure of mouse IgG2b Fc complexed with B domain of Protein A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2014-06-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 145.280, 145.280, 155.528 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.980 - 3.300 |
R-factor | 0.2102 |
Rwork | 0.208 |
R-free | 0.25030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rgs |
RMSD bond length | 0.011 |
RMSD bond angle | 1.317 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.530 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.080 | 0.884 |
Number of reflections | 25631 | 4547 |
<I/σ(I)> | 26.1 | 3.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 14.4 | 14.6 |
CC(1/2) | 0.999 | 0.936 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 60% (v/v) Tacsimate (pH 7.0) |